Next Generation Biomonitoring: Part Two, Volume 59, the latest release in the Advances in Ecological Research series, is the second part of a thematic on ecological biomonitoring. It includes specific chapters that cover aquatic volatile metabolomics using trace gases to examine ecological processes, next generation approaches to rapid monitoring Bio-aerosol and the link between human health and environmental microbiology, NGB in Canadian wetlands, CELLDEX/global monitoring of functional responses, Citizen Science and Biomonitoring, and more.