The technique of in situ hybridization is now vital to the molecular b iologists and their understanding of pathophysiology of cellular funct ions. This practical guide covers all aspects of in situ hybridization, describing: practical procedures and protocols, the scientific backg round, areas of application, and the limitations of the technology. Th is new edition has been completely rewritten by the experts in the fie ld to take account the many advances in the last seven years. Each cha pter contains up-to-date references, the latest advances and prospects for the future. Invaluable to molecular, cell and developmental biolo gists, pathologists and other clinical researchers, biologists and mic robiologists and all those interested in pathophysiology of cell funct ions and its regulation.