This textbook provides a strong foundation in biofilm engineering for graduate students from chemistry, microbiology, and engineering backgrounds. This text also serves as a valuable tool for faculty who are interested in introducing new courses on biofilm engineering, and as a useful supplement for undergraduate students. The book covers a wide range of topics, including basic biofilm concepts, engineering biofilms, imaging biofilms, the analysis of biofilm activity, biofilms applications, and negative impacts of biofilms, such as biocorrosion and disease.