1. Purification of RNA Expressed in vivo Inserted in a tRNA Scaffold Luc Ponchon and Frédéric Dardel 2. Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold Luc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte and Frédéric Dardel 3. Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR Jessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy, and Loren D. Williams 4. General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGE Jo L. Linpinsel and Graeme L. Conn 5. Preparation of Short RNA by in vitro Transcription Cheng Lu and Pingwei Li 6. Native RNA Purification by Gel Filtration Chromatography Evan P. Booy, Hui Meng and Sean A. McKenna 7. Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5' and 3' Ends Johanna M. Avis, Graeme L. Conn, and Scott C. Walker 8. Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' Ends Milena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski, and Jerzy Ciesiolka 9. Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme Methods Hae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong 10. Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA Geneviève Di Tomasso, Philipe Lampron, James G. Omichinski and Pascale Legault 11. Affinity Purification of RNA using an ARiBo Tag Geneviève Di Tomasso, Pierre Dagenais, Alexandre Desjardins, Alexis Rompré-Brodeur, Vanessa Delfosse and Pascale Legault 12. Plasmid Template Design and in vitro Transcription of Short RNAs within a 'Structure Cassette' for Structure Probing Experiments Virginia K. Vachon and Graeme L. Conn 13. In Vitro Transcription of Modified RNAs Stephanie L. Moon and Jeffrey Wilusz 14. End-Labeling Oligonucleotides with Chemical Tags After Synthesis N. Ruth Zearfoss and Sean P. Ryder 15. High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA Ya-Ming Hou 16. Se-derivatized RNAs for X-ray Crystallography Lina Lin and Zhen Huang 17. Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAs Luigi Martino and Maria R Conte 18. Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC Pierre Dagenais and Pascale Legault 19. Splint Ligation of RNA with T4 DNA Ligase Christopher J. Kershaw and Raymond T. O'Keefe