1. Introduction: Why Analyze Single Cells?Dino Di Carlo, Henry Tat Kwong Tse, and Daniel R. Gossett Part I: Conventional Methods 2. Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview Sara Lindström 3. Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry Robert B. Brown, Johannes A. Hewel, Andrew Emili, and Julie Audet 4. Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts Ward De Spiegelaere, Muriel Filliers, and Ann Van Soom Part II: Miniaturized Solutions 5. Single-Cell Culture in Microwells Sara Lindström and Helene Andersson-Svahn 6. Iso-Dielectric Separation and Analysis of Cells Michael D. Vahey and Joel Voldman 7. Single Cell Electroporation Using Microfluidic Devices Séverine Le Gac and Albert van den Berg 8. Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Build-In Pillar Array Chi Zhang 9. Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay Sara Henriksson and Mats Nilsson 10. Droplet Microfluidics for Single-Cell Analysis Eric Brouzes 11. Screening of Antigen-Specific Antibody Secreting Cells Hiroyuki Kishi, Aishun Jin, Tatsuhiko Ozawa, Kazuto Tajiri, Tsutomu Obata, and Atsushi Muraguchi 12. Analysis of Single Eukaryotic Cells Using Raman Tweezers Elsa Correia Faria and Peter Gardner 13. Single-Cell Microinjection Technologies Yan Zhang 14. Ultrasonic Manipulation of Single Cells Martin Wiklund and Björn Önfelt Part III: Reviews on Chosen Subjects Within the Field 15. Expanding the Horizons for Single-Cell Applications on Lab-On-a-Chip Devices Soo Hyeon Kim, Dominique Fourmy, and Teruo Fujii 16. Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses Ayça Yalçın, Yvonne J. Yamanaka, and J. Christopher Love