Table of Contents "Recombinant Gene Expression: Reviews and Protocols, Third Edition"Argelia Lorence (Editor) Name of corresponding author(s) is underlined General Aspects1) Using Folding Promoting Agents in Recombinant Protein Production: A Review Beatrix Fahnert 2) Routine Identity Confirmation of Recombinant Proteins by MALDI-TOF Mass SpectrometryBrett J. Savary and Prasanna Vasu 3) A Matter of Packaging: Influence of Nucleosome Positioning on Heterologous Gene ExpressionMaría de la Cruz Muñoz-Centeno, Gonzalo Millán-Zambrano and Sebastián Chavez 4) Tools of the Trade: Developing Antibody-Based Detection Capabilities for Recombinant ProteinsMaureen C. Dolan, Giuliana Medrano, Anthony McMickle, and Carole L. Cramer Prokaryotes 5) Heat-shock Protein Fusion Vectors for Improved Expression of Soluble Recombinant Proteins in Escherichia coli Christos A. Kyratsous and Christos A. Panagiotidis 6) The use of a Flagellar Export Signal for the Secretion of Recombinant Proteins in SalmonellaFerenc Vonderviszt, Rachel Sajo, Jozsef Dobo, Peter Zavodoszky 7) Optimization of Purification Protocols Based on the Step-by-step Monitoring of the Protein Aggregates in the Soluble FractionsArio De Marco 8) Heterologous Protein Expression by Lactococcus lactisJulio Villatoro-Hernández, Oscar Paul Kuipers, Odila Saucedo-Cárdenas and Roberto Montes de Oca Luna 9) An Extended Suite of Genetic Tools for Use in Bacteria of the Halomonadaceae: An Overview Monserrat Argandoña, Carmen Vargas, Javier Rodríguez-Moya, Mercedes Reina-Bueno, Manuel Salvador and Joaquin J. Nieto 10) Regulated Recombinant Protein Production in the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125Valentina Rippa, Rosanna Papa, Maria Giuliani, Cinzia Pezzella, Ermenegilda Parrilli, Maria Luisa Tutino, Gennaro Marino, and Angela Duilio 11) A Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125Maria Giuliani, Ermenegilda Parrilli , Cinzia Pezzella, Valentina Rippa, Angela Duilio, Gennaro Marino and Maria Luisa Tutino 12) A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein-protein InteractionJumei Zeng and Zheng-Guo He 13) Heterologous High-level Gene Expression in the Photosynthetic Bacterium Rhodobacter capsulatusNadine Katzke, René Bergmann, Karl-Erich Jaeger Thomas Drepper 14) Plasmid DNA Production for Therapeutic ApplicationsAlvaro Lara and Octavio T. Ramirez Lower eukaryotes 15) Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A ReviewTomoaki Niimi 16) Expression of Multi-subunit Proteins in Leishmania tarentolae Marisa Sugino and Tomoaki Niimi Fungi 17) Recombinant Protein Production in YeastsDiethard Mattanovich, Paola Branduardi, Laura Dato, Brigitte Gasser, Michael Sauer and Danilo Porro 18) Yeasts as Tool for Heterologous Gene ExpressionRaja Mokdad-Gargouri, Salma Abdelmoula-Soussi, Nadia Hadiji-Abbès, Ines Yacoubi-Hadj Amor, Istabrak Borchani-Chabchoub and Ali Gargouri 19) The Cre/lox system: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal EndophytesSimona Florea, Caroline Machado, Kalina Andreeva, and Christopher L. Schardl 20) Aptamer-regulated Expression