1. SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS Melanie Thelen, Dominic Winter, Thomas Braulke, and Volkmar Gieselmann 2. Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics Mesut Bilgin, Jesper Nylandsted, Marja Jäättelä, and Kenji Maeda 3. Analysis of N- And O-Glycosylation of Lysosomal Glycoproteins Elmira Tokhtaeva, Olga A. Mareninova, Anna S. Gukovskaya, and Olga Vagin 4. Analyzing Lysosome Related Organelles By Electron Microscopy Ilse Hurbain, Maryse Romao, Ptissam Bergam, Xavier Heiligenstein, And Graça Raposo 5. Microscopic Analysis of Lysosomal Membrane Permeabilization Ana Maria Vilamill Giraldo, Karin Öllinger, and Vesa Loitto 6. Quantitative Co-localization and Pattern Analysis Of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets Frederik W. Lund and Daniel Wüstner 7. Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues Mingguang Ren, Beibei Deng, Xiuqi Kong, Yonghe Tang, and Weiying Lin 8. Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy Dependent Recovery Takanobu Otomo and Tamotsu Yoshimori 9. Delivery of Cargo to Lysosomes using Gneosomes Kristina M. Hamill, Ezequiel Wexselblatt, Wenyong Tong, Jeffrey D. Esko, and Yitzhak Tor 10. Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles Camilla Lööv and Anna Erlandsson 11. Analysis of Lysosomal pH by Flow Cytometry Using FITC-dextran Loaded Cells Ida Eriksson, Karin Öllinger, and Hanna Appelqvist 12. Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry Anna L. Södergren and Sofia Ramström 13. Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes Norma W. Andrews 14. Using the MEROPS Database for Investigation of Lysosomal Peptidases, their Inhibitors and Substrates Neil D. Rawlings 15. Next Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of Lysoplex Giuseppina Di Fruscio, Sandro Banfi, Vincenzo Nigro, and Andrea Ballabio 16. Gelatin Zymography using Leupeptin for the Detection of Various Cathepsin L Forms Yoko Hashimoto 17. Methods for Determination of Α-Glycosidase, Β-Glycosidase, and Α-Galactosidase Activities in Dried Blood Spot Samples Eser Yıldırım Sozmen and Ebru Demirel Sezer 18. Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi Jyotsna Vermaa, Sunita Bijarnia-Mahaya, and Ishwar C. Vermaa 19. Lysosomal Biology in Cancer Colin Fennelly and Ravi Amaravadi