Preface... Table of Contents... Contributing Authors... 1. 'A Future Amalgamation between the Scientist and the Clinician?' Sarah Haywood-Small 2. Fresh Frozen Versus Formalin Fixed Paraffin Embedded for Mass Spectrometry Imaging Ekta Patel 3. Imaging MS of Rodent Ocular Tissues and the Optic Nerve David M.G. Anderson, Wendi Lambert, David J. Calkins, Szolt Abloczy, Rosalie K. Crouch, Richard M. Caprioli, and Kevin L. Schey 4. MALDI-MSI of Lipids in Human Skin Philippa J. Hart and Malcolm R. Clench 5. MALDI-MSI Analysis of Cytological Smears: The Study of Thyroid Cancer Niccolò Mosele, Andrew Smith, Manuel Galli, Fabio Pagni, and Fulvio Magni 6. Droplet-Based Liquid Extraction for Spatially-Resolved Microproteomics Analysis of Tissue Sections Maxence Wisztorski, Jusal Quanico, Julien Franck, Benoit Fatou, Michel Salzet, and Isabelle Fournier 7. DESI Mass Spectrometry Imaging (MSI) Emmanuelle Claude, Emrys Jones, and Steven Pringle 8. Peptide Imaging: Maximizing Peptide Yield, Optimization of the 'Peptide Mass Fingerprint' Ekta Patel 9. MALDI-MS Imaging in the Study of Glomerulonephritis Andrew Smith, Manuel Galli, Vincenzo L'Imperio, Fabio Pagni, and Fulvio Magni 10. Hierarchical Cluster Analysis to Aid Diagnostic Image Data Visualization of MS and Other Medical Imaging Modalities Arul N. Selvan, Laura M. Cole, Lynne Spackman, Sarah Naylor, and Chris Wright 11. Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Plant Metabolites Callie Seaman 12. Mass Spectrometry Imaging of Drugs of Abuse in Hair Bryn Flinders, Eva Cuypers, Tiffany Porta, Emmanuel Varesio, Gérard Hopfgartner, and Ron M.A. Heeren 13. MALDI Mass Spectrometry Profiling and Imaging Applied to the Analysis of Latent Fingermarks R. Bradshaw 14.ToF-SIMS Parallel Imaging MS/MS of Lipid Species in Thin Tissue Sections Anne L. Bruinen 15. Rodent Whole-Body Sectioning and MALDI Mass Spectrometry Imaging Paul J. Trim 16. The Future in Disease Models for Mass Spectrometry Imaging, Ethical Issues and the Way Forward Rebecca E. Day and Ieva Palubeckaite