Part I: General Toxicology 1. Nonclinical Development of Combination Drugs Alberto Lodola 2. Juvenile Non-Clinical Safety Studies in Support of Pediatric Drug Development Paul C. Barrow and Georg Schmitt Part II: Pathology 3. Procedures of Necropsy and Tissue Sampling Laurence Fiette, Mohamed Slaoui, and Anne-Laure Bauchet 4. Tissue Sampling and Processing for Histopathology Evaluation Mohamed Slaoui, Anne-Laure Bauchet, and Laurence Fiette 5. Principles and Methods of Immunohistochemistry José A. Ramos-Vara 6. Applications of Mass Spectrometry Imaging for Safety Evaluation David Bonnel and Jonathan Stauber Part III Genetic Toxicology 7. In vivo rat T-lymphocyte Pig-a assay: detection and expansion of cells deficient in the GPI-anchored CD48 surface marker for analysis of mutation in the endogenous Pig-a gene Vasily N Dobrovolsky, Javier Revollo, Dayton M Petibone, and Robert H Heflich 8. Detection of In Vivo Mutation in the Pig-a Gene of Mouse Bone Marrow Erythroids Takafumi Kimoto and Daishiro Miura 9. The Use of Bacterial Repair Endonucleases in the Comet Assay Andrew R. Collins Part IV Safety Pharmacology 10. Automated Patch-Clamp methods for the hERG Cardiac Potassium Channel Sylvie Houtmann, Brigitte Schombert, Camille Sanson, Michel Partiseti, and G. Andrees Bohme 11. Impedance Measurement in Induced Pluripotent Stem Cell Derived Cardiomyocytes Emma-Jane Poulton Part V Investigative Toxicology 12. Target Safety Assessment: Strategies and Resources Richard J. Brennan 13. NMR and MS Methods for Metabolomics Alexander Amberg, Björn Riefke, Götz Schlotterbeck, Alfred Ross, Hans Senn, Frank Dieterle, and Matthias Keck 14. Protocols and Applications of Cellular Metabolomics in Safety Studies Using Precision-Cut Tissue Slices and Carbon 13 NMR Gabriel Baverel, Maha El Hage, and Guy Martin 15. Statistical Analysis of Quantitative RT-PCR Results Richard Khan-Malek and Ying Wang 16. Evaluation of Mitochondrial Respiration in Cultured Rat Hepatocytes Jean-Pierre Marchandeau and Gilles Labbe Part VI Screening Assays for Developmental Toxicity 17. FETAX Assay for Evaluation of Developmental Toxicity Isabelle Mouche, Laure Malésic, and Olivier Gillardeaux 18. Evaluation of Embryotoxicity Using