Provides concise yet complete knowledge on the many aspects of the most useful yet the most controversial scientific techniques developed under the umbrella of genetic engineering, including detection, isolation and quantitation of DNA and RNA; enzymes of recombinant DNA technology and genome editing; cloning vectors; DNA cloning; DNA libraries; identification of gene of interest; analysis of cloned gene; plant marker gene systems; gene transfer through natural means; vectorless methods of gene transfer; gene transfer methods in plants; gene transfer methods in animals; assessment of gene transfer methods; RNA interference technology in designing transgenic crops; chloroplast genetic engineering; mitochondrial engineering; metabolic engineering; clean gene technology; genetic use restriction technology; mapping and cloning of genetic determinants of QTLs; applications and benefits of genetic engineering; Issues and disadvantages of genetic engineering; risk assessment of genetically engineered organisms; and some recent approaches, namely, genome engineering and genomically recoded organisms, currently being developed for production of safer genetically modified organisms. This book is primarily designed as a text book for undergraduate and graduate students studying genetic engineering in agricultural, veterinary and medicinal universities. Teachers and researchers in any discipline of life sciences, agricultural sciences, medicine, and biotechnology in all the conventional and agricultural universities, research institutes, molecular genetics and biotechnology companies/colleges/schools all over the world will also find it useful as a reference book.