Lipidomics is one of the newest 'omics' techniques with growing importance in bioscience. This book discusses interesting standard and non-standard techniques relevant to the measurement and analysis of lipids by mass spectrometry. It provides a guide to the possibilities of the techniques and introduces the reader to exciting newer methods which allow isomer differentiation, improve sensitivity, allow spatial location and go beyond annotation of simply matching a mass to a database entry. For the first time in a book, the emerging methods and advantages and disadvantages of new technologies for lipid structure characterization are highlighted.