Enzymes have made a marked impact on today's high technological society and are the central theme around which most biotechnology revolves. Enzymes being efficient as biocatalysts and superior to conventional chemical catalysts are being increasingly employed as replacements or additions for varied applications in various industries. Enzymes have made a marked impact on today's high technological society and are the central theme around which most biotechnology revolves. Enzymes being efficient as biocatalysts and superior to conventional chemical catalysts are being increasingly employed as replacements or additions for varied applications in various industries. The development of microbial enzymes for commercial use is a specialized field which requires. 1. Continuous screening of new and improved strains from potential sources. 2. Scaling up of enzyme production by optimizing conditions of fermentation. 3. Strain improvement of the isolated strains by classics or by recombinant methods of genetic manipulation. 4. Down stream purification technology and formulation of enzymes.