This book contains a collection of in vitro and in vivo studies that are relevant to Rife technology, with their analysis. It explains the fourth Rife effect, which is ionic, and how we can exploit it to improve the effectiveness of a Rife machine almost doubling it. The cure of some common ailments is also discussed and documented. Bacterial inactivation, apoptosis of cancer cells, accelerated wound healing are well documented and the concept of a new improved Rife machine is introduced